BioTech | NIT Sikkim

NameDr. Amlan Das
DesignationAssistant Professor
Academic Qualification2001-2003 : M. Sc in Biochemistry from Calcutta University.

2011: PhD, Department of Biotechnology, Calcutta University
Areas of InterestCancer stem cells, Oxidative stress, Antioxidant therapeutics
Work ExperiencePostdoctoral Research at Department of Life Sciences and Biotechnology, Jadavpur University as D. S. Kothari Postdoctoral Fellow (2016-2017).

Postdoctoral Research at VA Medical Center, Kansas City (2012-2015)

Postdoctoral Research at Ohio State University (2011-2012)

Doctoral Research at Department of Biotechnology, Calcutta University (2005-2010)
TeachingsMolecular Biology, Spectroscopy, Biophysical Chemistry, Cell Biology
List of PublicationsPeer-Reviewed Publications
  1. Sarkar S, Ghosh A, Banerjee S, Maity G, Das A, Larson MA , Gupta V, Haque I, Tawfik O and Banerjee SK . CCN5/WISP-2 restores ER-? in normal and neoplastic breast cells and sensitizes triple negative breast cancer cells to tamoxifen. Oncogenesis [Nature publishing group] 2017 (Accepted).
  2. Das A, Dhar K, Maity G, Sarkar S, Ghosh A, Haque I, Dhar G, Banerjee S and Banerjee S. K. Deficiency of CCN5/WISP-2-Driven Program in breast cancer Promotes Cancer Epithelial cells to mesenchymal stem cells and Breast Cancer growth. Scientific Reports [Nature publishing group], 2017 ( Accepted).
  3. Datta S, Choudhury D, Das A, Das Mukherjee D, Das N, Roy SS, Chakrabarti G. Paclitaxel resistance development is associated with biphasic changes in reactive oxygen species, mitochondrial membrane potential and autophagy with elevated energy production capacity in lung cancer cells: A chronological study. Tumour Biol. 2017 Feb;39(2):1010428317694314.
  4. Das Mukherjee D, Kumar NM, Tantak MP, Das A, Ganguli A, Datta S, Kumar D, Chakrabarti G. Development of novel bis(indolyl)-hydrazide-hydrazone derivatives as potent microtubule- targeting cytotoxic agents against A549 lung cancer cells. Biochemistry. 2016 55(21):3020-35. doi: 10.1021/acs.biochem.5b01127.
  5. Bhattacharya S, Das A, Datta Choudhury S and Chakrabarti S. Colchicine induces autophagy and senescence in lung cancer cells at clinically admissible concentration: potential use of colchicine in combination with autophagy inhibitor in cancer therapy. Tumor Biology 2016 [Epub ahead of print].
  6. Headley CA, DiSilvestro D, Bryant KE, Hemann C, Chen CA, Das A, Ziouzenkova, Durand G, Villamena F. Nitrones Reverse Hyperglycemia-Induced Endothelial Dysfunction in Bovine Aortic Endothelial Cells. Biochem Pharmacology 2016, 104:108-17. doi: 10.1016/j.bcp.2016.01.005.
  7. Ganguli A, Das A, Nag D, Bhattacharya S, Chakrabarti S. The dietary flavonoid morin confers protection to oral epithelial cells form smokeless tobacco extract (STE)-mediated cytotoxicity by attenuating STE-induced autophagy. Food Chem Toxicology 2016, 90:160-70. doi: 10.1016/j.fct.2016.02.011..
  8. Chakrabarty S, Ganguli A, Das A, Nag D, Chakrabarti G Epigallocatechin-3-gallate shows anti-proliferative activity in HeLa Cells targeting tubulin-microtubule equilibrium. Chemico-Biological Interactions 2015, 242:380-9.
  9. Maity G, De A, Das A, Banerjee S, Sarkar S, and Banerjee SK Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition. Lab Invest. 2015; 702-17. Apr 13. doi: 10.1038/labinvest.2015.49.
  10. Rosselin M, Choteau F, Zeamari K, Nash KM, Das A, Lauricella R, Lojou E, Tuccio B, Villamena FA, and Durand G, Reactivities of Substituted ?-Phenyl-N-tert-butyl Nitrones, J Org Chem. 2014 ; 79(14):6615-26. doi: 10.1021/jo501121g.
  11. Das, A, Gopalakrishnan,B, Druhan, L, Wang, T Y, De Pascali, F, Racoma, I, Varadharaj, S, Zweier, J, Cardounel, A, Rockenbauer, A and Villamena F , Reversal of SIN-1-induced eNOS dysfunction by the spin trap, DMPO, in bovine aortic endothelial cells via eNOS phosphorylation, British Journal of Pharmacology, 2014; 171(9):2321-34. doi: 10.1111/bph.12572.
  12. Chakraborti S, Dhar G, Dwivedi V, Das A, Poddar A, Chakraborti G, Basu G, Chakrabarti P, Surolia A, Bhattacharyya B. Stable and potent analogues derived from the modification of the dicarbonyl moiety of curcumin. Biochemistry. 2013, 52(42):7449-60. doi: 10.1021/bi400734e.
  13. Das A, Bhattacharya A, Chakbabarty S and Chakrabarti G, Smokeless tobacco extract (STE)-induced toxicity in mammalian cells is mediated by the disruption of cellular microtubule network: a key mechanism of cytotoxicity. Plos One, 2013, 8(7):e68224. doi: 10.1371/journal.pone.0068224.
  14. Perez-Cruz F, Vazquez-Rodriguez S, Matos MJ, Herrera-Morales A, Villamena FA, Das A, Gopalakrishnan B, Olea-Azar C, Santana L, Uriarte E Synthesis, electrochemical and biological studies on novel coumarin-chalcone hybrid compounds. J. Med Chem. 2013, ;56(15):6136-45. doi: 10.1021/jm400546y
  15. Fernanda Perez-Cruz, Frederick A. Villamena, Gerald Zapata-Torres, Amlan Das, Colwyn A. Headley, Elias Quezada, Camilo Lopez-Alarcon, Claudio Olea-Azar, Selected hydroxycoumarins as antioxidants in cells: physicochemical and reactive oxygen species scavenging studies. J. Org. Physical Chemistry. 2013.
  16. Choudhury D, Ganguli A, Dastidar DG, Acharya BR, Das A, Chakrabarti G, Apigenin synergistically enhances anticancer activity of curcumin by co-targeting tubulin-microtubule system; a novel therapeutic approach towards new anticancer drug with negligible side effects Biochimie . 2013 Feb 26. doi:pii: S0300-9084(13)00067-9. 10.1016/j.biochi.2013.02.010.
  17. Kambhampati S, Rajewski RA, Tanol M, Haque I, Das A, Banerjee S, Jha S, Burns D, Borrego-Diaz E, Van Veldhuizen P, Banerjee SK.A Second Generation 2-Methoxyestradiol Prodrug is Effective against Barrett's Adenocarcinoma in a Mouse Xenograft Model. Mol Cancer Ther. 2013 Jan, 12(3):255-63. (Cover Page figure, Immunofluorescence done by A Das).
  18. Das, A.; Choudhury, D.; Chakrabarty, S.; Bhattacharya, A.; Chakrabarti, G "Acenaphthenequinone induces cell cycle arrest and mitochondrial apoptosis via disruption of cellular microtubule." Toxicological Research (Royal Society of Chemistry ) 2012,1, 171-185. DOI: 10.1039/C2TX00013J (Cover Page figure)
  19. Villamena FA; Das A; Nash KM. "Potential implication of the chemical properties and bioactivity of nitrone spin traps for therapeutics." Future Med Chem. ( Future Science) 2012 June;4(9):1171-207
  20. Das A; Gopalakrishnan B; Voss OH; Doseff AI; Villamena FA. "Inhibition of ROS-induced apoptosis in endothelial cells by nitrone spin traps via induction of phase II enzymes and suppression of mitochondria-dependent pro-apoptotic signaling." Biochem Pharmacol. (Elsevier) 2012 May, 84(4):486-97.
  21. Chakraborti , S.; Das, L; Kapoor, N; Das, A.; Poddar, A.; Chakrabarti, G.; Janik, M.; Basu, G; Panda, D; Chakrabarti, P.; Surolia, A.; Bhattacharyya B "Curcumin recognizes a unique binding site of Tubulin" Journal of Medicinal Chemistry (American Chemical Society) 2011, Sep 22;54(18):6183-96
  22. Chakrabarty, S.; Das, A.; Bhattacharya, A.; Chakrabarti, G "Theaflavins depolymerize microtubule network through tubulin binding and cause apoptosis of cervical carcinoma HeLa cells." Journal of Agricultural and Food Chemistry. (American Chemical Society) 2011. 59:2040-2048.
  23. Choudhury, D.; Das, A.; Bhattacharya, A.; Chakrabarti, G. "Aqueous extract of ginger shows antiproliferative activity through disruption of microtubule network of cancer cells." Food Chem Toxicol. (Elsevier) 2010. 48 :2872-2880 .
  24. Das, A.; Chakrabarty, S.; Choudhury, D.; Chakrabarti, G. "1,4-Benzoquinone (PBQ) induced toxicity in lung epithelial cells is mediated by the disruption of the microtubule network and activation of caspase-3." Chem Res Toxicol. (American Chemical Society) 2010. 23 :1054-1066.
  25. Acharya, B. R; Choudhury, D.; Das, A; Chakrabarti, G. "Vitamin K3 disrupts the microtubule networks by binding to tubulin: a novel mechanism of its antiproliferative activity." Biochemistry. (American Chemical Society) 2009 48:6963-6974.
  26. Das, A.; Bhattacharya, A.; Chakrabarti, G. "Cigarette Smoke Extract Induces Disruption of Structure and Function of Tubulin-Microtubule in Lung Epithelium Cells and in Vitro." Chem Res Toxicol. (American Chemical Society) 2009 22, 446-459.
  27. Das, A.; Sinha, S.; Acharya, B. R.; Paul, P.; Bhattacharyya, B.; Chakrabarti, G. "Deuterium oxide stabilizes conformation of tubulin:a biophysical and biochemical study." BMB Rep. (Korean Society for Biochemistry and Molecular Biology) 2008. 41:62-67.
  28. Sahoo, R.; Dutta, T.; Das, A.; Sinha, Ray, S.; Sengupta, R.; Ghosh S. "Effect of nitrosative stress on Schizosaccharomyces pombe: inactivation of glutathione reductase by peroxynitrite." Free Radic Biol Med. (Elsevier) 2006. 40 :625-6231
Membership of Professional Bodies
  1. American Society for Biochemistry and Molecular Biology (ASBMB)
  2. American Society for Cancer Research (AACR)
AchievementsAwards and Fellowships
  1. 2005 University Research Fellowship, Calcutta University.
  2. 2009 Best Poster Award in "7th COSTAM/SFRR (ASIA/MALAYSIA) INTERNATIONAL WORKSHOP", SFRR, Malaysia
  3. 2009 "Best Free Radical Research in Health", SFRR, Asia
  4. 2014 Travel Award Fellowship by American
Society for Biochemistry and Cell Biology, ASBMB Meeting, Boston, USA
  1. 2016 Dr. D S Kothary Postdoctoral Fellowship for Research, UGC, India.
  2. 2016 Best Poster Presentation Award in meeting organized by Asian Society of Clinical Oncology, New Delhi, India.
  3. 2017 Best Poster Award, 3rd International Conference on. Perspective of Cell Signaling and Molecular Medicine, Bose Institute, Kolkata, India.
Office AddressDepartment of Biotechnology, NIT Sikkim Ravangla Campus, Barfung Block Sikkim 737139